Review



polyclonal goat igg anti mouse cd4 antibody  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    R&D Systems polyclonal goat igg anti mouse cd4 antibody
    Multiparameter spectral flow cytometry analysis of immune cells of the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in <xref ref-type=Supplementary Figure 1 . (A) Automated T-distributed stochastic neighbour embedding (t-SNE) 2D map of the flow cytometry data acquired from control and infected mice colon. (B) Upper panel; tSNE 2D map showing scaled expression of CD11b for myeloid cells, B220 for B cells and CD3 for T cells. Lower panel; tSNE 2D map showing the location of CD11b + myeloid cells, B220 + B cells and CD3 + T cells. (C) Heat maps for (left) cell surface markers expression (CD45, CD11b, B220, CD3, CD4 and CD8) and (right) groups (control, acute and chronic colons) for 22 cell clusters identified. (D) Percentage of each cluster for each group. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Arrows in (C) and (D) indicate immune cell clusters of myeloid cells (blue), B cells (green) or T cells (red). " width="250" height="auto" />
    Polyclonal Goat Igg Anti Mouse Cd4 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+anti+cd4/pmc13038538-114-2-8?v=R%26D+Systems
    Average 93 stars, based on 63 article reviews
    polyclonal goat igg anti mouse cd4 antibody - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease"

    Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2026.1761769

    Multiparameter spectral flow cytometry analysis of immune cells of the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in <xref ref-type=Supplementary Figure 1 . (A) Automated T-distributed stochastic neighbour embedding (t-SNE) 2D map of the flow cytometry data acquired from control and infected mice colon. (B) Upper panel; tSNE 2D map showing scaled expression of CD11b for myeloid cells, B220 for B cells and CD3 for T cells. Lower panel; tSNE 2D map showing the location of CD11b + myeloid cells, B220 + B cells and CD3 + T cells. (C) Heat maps for (left) cell surface markers expression (CD45, CD11b, B220, CD3, CD4 and CD8) and (right) groups (control, acute and chronic colons) for 22 cell clusters identified. (D) Percentage of each cluster for each group. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Arrows in (C) and (D) indicate immune cell clusters of myeloid cells (blue), B cells (green) or T cells (red). " title="... cell surface markers expression (CD45, CD11b, B220, CD3, CD4 and CD8) and (right) groups (control, acute and ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Multiparameter spectral flow cytometry analysis of immune cells of the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in Supplementary Figure 1 . (A) Automated T-distributed stochastic neighbour embedding (t-SNE) 2D map of the flow cytometry data acquired from control and infected mice colon. (B) Upper panel; tSNE 2D map showing scaled expression of CD11b for myeloid cells, B220 for B cells and CD3 for T cells. Lower panel; tSNE 2D map showing the location of CD11b + myeloid cells, B220 + B cells and CD3 + T cells. (C) Heat maps for (left) cell surface markers expression (CD45, CD11b, B220, CD3, CD4 and CD8) and (right) groups (control, acute and chronic colons) for 22 cell clusters identified. (D) Percentage of each cluster for each group. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Arrows in (C) and (D) indicate immune cell clusters of myeloid cells (blue), B cells (green) or T cells (red).

    Techniques Used: Flow Cytometry, Infection, Isolation, Control, Expressing, Comparison

    Phenotypic analysis of T cells via multiparameter spectral flow cytometry in the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in <xref ref-type=Supplementary Figure 1 . (A) T cells composition by flow cytometry, (B) tSNE 2D map showing scaled expression of CD3, CD4, and CD8 cell makers (C) Heat map of T cells (CD45 + , CD3 + ) (highlighted clusters 1, 7, 9, 16, 20, 22). Arrows indicate immune cell clusters of inflammatory (purple) and regulatory (pink) double-negative (DN) T cells. (D) tSNE 2D map of DN T cells and percentage of T cells cluster for each group. Arrows indicate DN T cell clusters of inflammatory (purple) and regulatory (pink) cells. (E) Percentage of DN T cells with inflammatory/regulatory phenotypes in total immune cells (CD45 + cells). (F) Relative percentage of DN T cells with inflammatory/regulatory phenotypes in total DN T cells. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Bars in A represent mean ± SD, and individual symbols denote values from single mice (n = 15; 5 per set, 3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, ****p < 0.0001. " title="... tSNE 2D map showing scaled expression of CD3, CD4, and CD8 cell makers (C) Heat map of ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Phenotypic analysis of T cells via multiparameter spectral flow cytometry in the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in Supplementary Figure 1 . (A) T cells composition by flow cytometry, (B) tSNE 2D map showing scaled expression of CD3, CD4, and CD8 cell makers (C) Heat map of T cells (CD45 + , CD3 + ) (highlighted clusters 1, 7, 9, 16, 20, 22). Arrows indicate immune cell clusters of inflammatory (purple) and regulatory (pink) double-negative (DN) T cells. (D) tSNE 2D map of DN T cells and percentage of T cells cluster for each group. Arrows indicate DN T cell clusters of inflammatory (purple) and regulatory (pink) cells. (E) Percentage of DN T cells with inflammatory/regulatory phenotypes in total immune cells (CD45 + cells). (F) Relative percentage of DN T cells with inflammatory/regulatory phenotypes in total DN T cells. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Bars in A represent mean ± SD, and individual symbols denote values from single mice (n = 15; 5 per set, 3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, ****p < 0.0001.

    Techniques Used: Flow Cytometry, Infection, Isolation, Control, Expressing, Comparison

    Double-negative T cells phenotypes in the colonic lamina propria of C57BL/6 mice during T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Mice were euthanized during the acute (30 dpi) and chronic (90 dpi) phases. Colonic lamina propria cells were isolated from uninfected (Control; blue), acutely infected (Acute; pink) and chronically infected (Chronic; green) mice. Cells were gated on single cells, live, CD45 + , CD3 + , CD4 - , CD8 - events and subsequently on (A) inflammatory immune cell markers including CCR5, CXCR3 or Granzyme B, and (B) regulatory immune cells markers including CCR4, IL10Rα or IL10. Bars represent mean ± SD, and individual symbols denote values from single mice (n = 8-12; 4 per set, 2–3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, **p < 0.01, ***p < 0.001, **** p ≤ 0.0001.
    Figure Legend Snippet: Double-negative T cells phenotypes in the colonic lamina propria of C57BL/6 mice during T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Mice were euthanized during the acute (30 dpi) and chronic (90 dpi) phases. Colonic lamina propria cells were isolated from uninfected (Control; blue), acutely infected (Acute; pink) and chronically infected (Chronic; green) mice. Cells were gated on single cells, live, CD45 + , CD3 + , CD4 - , CD8 - events and subsequently on (A) inflammatory immune cell markers including CCR5, CXCR3 or Granzyme B, and (B) regulatory immune cells markers including CCR4, IL10Rα or IL10. Bars represent mean ± SD, and individual symbols denote values from single mice (n = 8-12; 4 per set, 2–3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, **p < 0.01, ***p < 0.001, **** p ≤ 0.0001.

    Techniques Used: Infection, Isolation, Control

    Microscopy analysis of T. cruzi infected colons. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc-RFP). Colons were isolated from control and chronically infected mice (90 dpi), processed for microscopy and stained with CD3 (Blue), CD4 (Green) and CD8 (Red) specific antibodies ( <xref ref-type=Supplementary Table 1 , Antibody Panel 4) as described in the materials and methods. Immunofluorescence images of cross sections of colons obtained from non-infected mice (Control) and chronically infected mice (Chronic) are shown in panels (A, B) , respectively. In (A, B) , a low magnification image of the entire colon is shown in the upper left, a region of interest (ROI) 1 is shown at mid-magnification in the lower left, and a high magnification image of ROI 2 is shown in shown on the right. For ROI 2, an overlay image of the 3 channels CD3 (Blue), CD4 (Green) and CD8 (Red) is shown on the top right, and the individual channels are represented below as indicated. A representative result of 3 controls and 3 infected colons is shown in this figure. LP, Lamina Propria; solid white arrow heads, double-negative T cells (CD3 + , CD4 - , CD8 - ); open arrowheads, CD3 - , CD4 - , CD8 + cells; solid pink arrowheads, CD3 - , CD4 + , CD8 - cells; solid yellow arrowheads, CD3 - , CD4 + , CD8 + cells. " title="... processed for microscopy and stained with CD3 (Blue), CD4 (Green) and CD8 (Red) specific antibodies (
    Figure Legend Snippet: Microscopy analysis of T. cruzi infected colons. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc-RFP). Colons were isolated from control and chronically infected mice (90 dpi), processed for microscopy and stained with CD3 (Blue), CD4 (Green) and CD8 (Red) specific antibodies ( Supplementary Table 1 , Antibody Panel 4) as described in the materials and methods. Immunofluorescence images of cross sections of colons obtained from non-infected mice (Control) and chronically infected mice (Chronic) are shown in panels (A, B) , respectively. In (A, B) , a low magnification image of the entire colon is shown in the upper left, a region of interest (ROI) 1 is shown at mid-magnification in the lower left, and a high magnification image of ROI 2 is shown in shown on the right. For ROI 2, an overlay image of the 3 channels CD3 (Blue), CD4 (Green) and CD8 (Red) is shown on the top right, and the individual channels are represented below as indicated. A representative result of 3 controls and 3 infected colons is shown in this figure. LP, Lamina Propria; solid white arrow heads, double-negative T cells (CD3 + , CD4 - , CD8 - ); open arrowheads, CD3 - , CD4 - , CD8 + cells; solid pink arrowheads, CD3 - , CD4 + , CD8 - cells; solid yellow arrowheads, CD3 - , CD4 + , CD8 + cells.

    Techniques Used: Microscopy, Infection, Isolation, Control, Staining, Immunofluorescence



    Similar Products

    polyclonal goat igg anti mouse cd4 antibody  (R&D Systems)
    93
      Buy from Supplier
    goat polyclonal anti cd4  (R&D Systems)
    94
      Buy from Supplier
    goat polyclonal antibody against sox2  (Abcam)
    99
    Abcam goat polyclonal antibody against sox2
    Cellular and gene expression analyses of stem cell activation elicited by sequential running stimuli in p16 KO and p16 WT dentate gyrus. (A) Timeline of the experiments performed on p16 WT and p16 KO 1-year-old mice: i) no run; ii) single run of 7 days; iii) single run of 12 days followed by a sedentary period; iv) double run of 12 and 7 days spaced 21 days apart. (B) Representative images by confocal microscopy (×40 magnification) showing that the proliferating type-1 stem cells (Ki67 + <t>/Sox2</t> + /GFAP + , labeled in red, blue and green, respectively) in the dentate gyrus of p16 KO mice increase, relative to p16 WT, after a single run of 12 days followed by a sedentary period, whereas they do not increase after a double run of 12 and 7 days spaced 21 days apart. The white dashed line labels the boundaries of the dentate gyrus. Arrowheads: Ki67 + /Sox2 + /GFAP − cells, arrows: Ki67 + /Sox2 + /GFAP + cells. Scale bar 50 μm. White boxes: area at higher magnification (×2), shown in the panels on the right. (C) Graph showing the changes induced by running on the proliferation of stem cells (type-1 cells, Ki67 + /Sox2 + /GFAP + ) in p16 WT and in p16 KO dentate gyrus, in the different groups indicated. A single RUN of 12 days induces the proliferation of p16 KO stem cells for an extended period (28 days; group 12d RUN +28d), but a second running added (7 days; group 12d RUN +21d + 7d RUN) does not stimulate p16 KO stem cell proliferation. (D) Similar changes were observed analyzing stem and progenitor cells together (i.e., type-1 and type-2a cells, respectively; Ki67 + /Sox2 + cells). (C,D) Analysis of simple effects: NS p > 0.05, ** p < 0.01, or **** p < 0.0001, PLSD ANOVA test. Cell numbers in the dentate gyrus are means ± SEM of the analysis of five animals per group. (E) Real-time PCR analysis of the differential expression response to the second running stimulus for the Set A_Set E_GLM genes whose activation by running had been validated, i.e., Tfap2c , Lepr , Top2a , Slc18a2 , Lpin2 , Rgs14 , Ramp3 , Chrm5 . The expression of these genes was activated in the p16 knockout dentate gyrus by a single run of 12 days but was not changed by a second running, relative to p16 wild-type. The figure shows the mean mRNA expression fold increases ±SEM from two independent experiments. Four biological replicates were used for mice that run 12 days and five biological replicates for mice that underwent the double run. TBP was used to normalize data. The statistical analysis of real-time PCR data was performed by non-parametric Kruskal-Wallis test to detect main effects (resulting with p -value <0.05 for all the genes tested), followed by Mann-Whitney U post hoc test for single Set comparison. * p < 0.05, ** p < 0.01, *** p < 0.001, Mann-Whitney U post hoc test; n = at least 24, i.e., total number of data for each gene analyzed for all four groups (WT RUN and KO RUN, WT 12d RUN +21d + 7d RUN or KO 12d RUN +21d + 7d RUN).
    Goat Polyclonal Antibody Against Sox2, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+anti+cd4/pmc10621069-246-24-29?v=Abcam
    Average 99 stars, based on 1 article reviews
    goat polyclonal antibody against sox2 - by Bioz Stars, 2026-07
    99/100 stars
    		  Buy from Supplier
    anti cd4  (OriGene)
    91
    OriGene anti cd4
    Cellular and gene expression analyses of stem cell activation elicited by sequential running stimuli in p16 KO and p16 WT dentate gyrus. (A) Timeline of the experiments performed on p16 WT and p16 KO 1-year-old mice: i) no run; ii) single run of 7 days; iii) single run of 12 days followed by a sedentary period; iv) double run of 12 and 7 days spaced 21 days apart. (B) Representative images by confocal microscopy (×40 magnification) showing that the proliferating type-1 stem cells (Ki67 + <t>/Sox2</t> + /GFAP + , labeled in red, blue and green, respectively) in the dentate gyrus of p16 KO mice increase, relative to p16 WT, after a single run of 12 days followed by a sedentary period, whereas they do not increase after a double run of 12 and 7 days spaced 21 days apart. The white dashed line labels the boundaries of the dentate gyrus. Arrowheads: Ki67 + /Sox2 + /GFAP − cells, arrows: Ki67 + /Sox2 + /GFAP + cells. Scale bar 50 μm. White boxes: area at higher magnification (×2), shown in the panels on the right. (C) Graph showing the changes induced by running on the proliferation of stem cells (type-1 cells, Ki67 + /Sox2 + /GFAP + ) in p16 WT and in p16 KO dentate gyrus, in the different groups indicated. A single RUN of 12 days induces the proliferation of p16 KO stem cells for an extended period (28 days; group 12d RUN +28d), but a second running added (7 days; group 12d RUN +21d + 7d RUN) does not stimulate p16 KO stem cell proliferation. (D) Similar changes were observed analyzing stem and progenitor cells together (i.e., type-1 and type-2a cells, respectively; Ki67 + /Sox2 + cells). (C,D) Analysis of simple effects: NS p > 0.05, ** p < 0.01, or **** p < 0.0001, PLSD ANOVA test. Cell numbers in the dentate gyrus are means ± SEM of the analysis of five animals per group. (E) Real-time PCR analysis of the differential expression response to the second running stimulus for the Set A_Set E_GLM genes whose activation by running had been validated, i.e., Tfap2c , Lepr , Top2a , Slc18a2 , Lpin2 , Rgs14 , Ramp3 , Chrm5 . The expression of these genes was activated in the p16 knockout dentate gyrus by a single run of 12 days but was not changed by a second running, relative to p16 wild-type. The figure shows the mean mRNA expression fold increases ±SEM from two independent experiments. Four biological replicates were used for mice that run 12 days and five biological replicates for mice that underwent the double run. TBP was used to normalize data. The statistical analysis of real-time PCR data was performed by non-parametric Kruskal-Wallis test to detect main effects (resulting with p -value <0.05 for all the genes tested), followed by Mann-Whitney U post hoc test for single Set comparison. * p < 0.05, ** p < 0.01, *** p < 0.001, Mann-Whitney U post hoc test; n = at least 24, i.e., total number of data for each gene analyzed for all four groups (WT RUN and KO RUN, WT 12d RUN +21d + 7d RUN or KO 12d RUN +21d + 7d RUN).
    Anti Cd4, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+anti+cd4/pmc10368451-43-44-45?v=OriGene
    Average 91 stars, based on 1 article reviews
    anti cd4 - by Bioz Stars, 2026-07
    91/100 stars
    		  Buy from Supplier
    gr320743 5 anti cd4 goat polyclonal igg r d systems  (R&D Systems)
    93
    R&D Systems gr320743 5 anti cd4 goat polyclonal igg r d systems
    Cellular and gene expression analyses of stem cell activation elicited by sequential running stimuli in p16 KO and p16 WT dentate gyrus. (A) Timeline of the experiments performed on p16 WT and p16 KO 1-year-old mice: i) no run; ii) single run of 7 days; iii) single run of 12 days followed by a sedentary period; iv) double run of 12 and 7 days spaced 21 days apart. (B) Representative images by confocal microscopy (×40 magnification) showing that the proliferating type-1 stem cells (Ki67 + <t>/Sox2</t> + /GFAP + , labeled in red, blue and green, respectively) in the dentate gyrus of p16 KO mice increase, relative to p16 WT, after a single run of 12 days followed by a sedentary period, whereas they do not increase after a double run of 12 and 7 days spaced 21 days apart. The white dashed line labels the boundaries of the dentate gyrus. Arrowheads: Ki67 + /Sox2 + /GFAP − cells, arrows: Ki67 + /Sox2 + /GFAP + cells. Scale bar 50 μm. White boxes: area at higher magnification (×2), shown in the panels on the right. (C) Graph showing the changes induced by running on the proliferation of stem cells (type-1 cells, Ki67 + /Sox2 + /GFAP + ) in p16 WT and in p16 KO dentate gyrus, in the different groups indicated. A single RUN of 12 days induces the proliferation of p16 KO stem cells for an extended period (28 days; group 12d RUN +28d), but a second running added (7 days; group 12d RUN +21d + 7d RUN) does not stimulate p16 KO stem cell proliferation. (D) Similar changes were observed analyzing stem and progenitor cells together (i.e., type-1 and type-2a cells, respectively; Ki67 + /Sox2 + cells). (C,D) Analysis of simple effects: NS p > 0.05, ** p < 0.01, or **** p < 0.0001, PLSD ANOVA test. Cell numbers in the dentate gyrus are means ± SEM of the analysis of five animals per group. (E) Real-time PCR analysis of the differential expression response to the second running stimulus for the Set A_Set E_GLM genes whose activation by running had been validated, i.e., Tfap2c , Lepr , Top2a , Slc18a2 , Lpin2 , Rgs14 , Ramp3 , Chrm5 . The expression of these genes was activated in the p16 knockout dentate gyrus by a single run of 12 days but was not changed by a second running, relative to p16 wild-type. The figure shows the mean mRNA expression fold increases ±SEM from two independent experiments. Four biological replicates were used for mice that run 12 days and five biological replicates for mice that underwent the double run. TBP was used to normalize data. The statistical analysis of real-time PCR data was performed by non-parametric Kruskal-Wallis test to detect main effects (resulting with p -value <0.05 for all the genes tested), followed by Mann-Whitney U post hoc test for single Set comparison. * p < 0.05, ** p < 0.01, *** p < 0.001, Mann-Whitney U post hoc test; n = at least 24, i.e., total number of data for each gene analyzed for all four groups (WT RUN and KO RUN, WT 12d RUN +21d + 7d RUN or KO 12d RUN +21d + 7d RUN).
    Gr320743 5 Anti Cd4 Goat Polyclonal Igg R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+anti+cd4/pm34820603-731-67-72?v=R%26D+Systems
    Average 93 stars, based on 1 article reviews
    gr320743 5 anti cd4 goat polyclonal igg r d systems - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier
    anti cd4 goat polyclonal igg  (R&D Systems)
    93
    R&D Systems anti cd4 goat polyclonal igg
    Cellular and gene expression analyses of stem cell activation elicited by sequential running stimuli in p16 KO and p16 WT dentate gyrus. (A) Timeline of the experiments performed on p16 WT and p16 KO 1-year-old mice: i) no run; ii) single run of 7 days; iii) single run of 12 days followed by a sedentary period; iv) double run of 12 and 7 days spaced 21 days apart. (B) Representative images by confocal microscopy (×40 magnification) showing that the proliferating type-1 stem cells (Ki67 + <t>/Sox2</t> + /GFAP + , labeled in red, blue and green, respectively) in the dentate gyrus of p16 KO mice increase, relative to p16 WT, after a single run of 12 days followed by a sedentary period, whereas they do not increase after a double run of 12 and 7 days spaced 21 days apart. The white dashed line labels the boundaries of the dentate gyrus. Arrowheads: Ki67 + /Sox2 + /GFAP − cells, arrows: Ki67 + /Sox2 + /GFAP + cells. Scale bar 50 μm. White boxes: area at higher magnification (×2), shown in the panels on the right. (C) Graph showing the changes induced by running on the proliferation of stem cells (type-1 cells, Ki67 + /Sox2 + /GFAP + ) in p16 WT and in p16 KO dentate gyrus, in the different groups indicated. A single RUN of 12 days induces the proliferation of p16 KO stem cells for an extended period (28 days; group 12d RUN +28d), but a second running added (7 days; group 12d RUN +21d + 7d RUN) does not stimulate p16 KO stem cell proliferation. (D) Similar changes were observed analyzing stem and progenitor cells together (i.e., type-1 and type-2a cells, respectively; Ki67 + /Sox2 + cells). (C,D) Analysis of simple effects: NS p > 0.05, ** p < 0.01, or **** p < 0.0001, PLSD ANOVA test. Cell numbers in the dentate gyrus are means ± SEM of the analysis of five animals per group. (E) Real-time PCR analysis of the differential expression response to the second running stimulus for the Set A_Set E_GLM genes whose activation by running had been validated, i.e., Tfap2c , Lepr , Top2a , Slc18a2 , Lpin2 , Rgs14 , Ramp3 , Chrm5 . The expression of these genes was activated in the p16 knockout dentate gyrus by a single run of 12 days but was not changed by a second running, relative to p16 wild-type. The figure shows the mean mRNA expression fold increases ±SEM from two independent experiments. Four biological replicates were used for mice that run 12 days and five biological replicates for mice that underwent the double run. TBP was used to normalize data. The statistical analysis of real-time PCR data was performed by non-parametric Kruskal-Wallis test to detect main effects (resulting with p -value <0.05 for all the genes tested), followed by Mann-Whitney U post hoc test for single Set comparison. * p < 0.05, ** p < 0.01, *** p < 0.001, Mann-Whitney U post hoc test; n = at least 24, i.e., total number of data for each gene analyzed for all four groups (WT RUN and KO RUN, WT 12d RUN +21d + 7d RUN or KO 12d RUN +21d + 7d RUN).
    Anti Cd4 Goat Polyclonal Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+anti+cd4/pm34820603-766-74-82?v=R%26D+Systems
    Average 93 stars, based on 1 article reviews
    anti cd4 goat polyclonal igg - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier
    goat polyclonal anti human cd4  (R&D Systems)
    94
    R&D Systems goat polyclonal anti human cd4
    Inclusion and exclusion criteria.
    Goat Polyclonal Anti Human Cd4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+anti+cd4/pmc08466465-174-21-26?v=R%26D+Systems
    Average 94 stars, based on 1 article reviews
    goat polyclonal anti human cd4 - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Multiparameter spectral flow cytometry analysis of immune cells of the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in <xref ref-type=Supplementary Figure 1 . (A) Automated T-distributed stochastic neighbour embedding (t-SNE) 2D map of the flow cytometry data acquired from control and infected mice colon. (B) Upper panel; tSNE 2D map showing scaled expression of CD11b for myeloid cells, B220 for B cells and CD3 for T cells. Lower panel; tSNE 2D map showing the location of CD11b + myeloid cells, B220 + B cells and CD3 + T cells. (C) Heat maps for (left) cell surface markers expression (CD45, CD11b, B220, CD3, CD4 and CD8) and (right) groups (control, acute and chronic colons) for 22 cell clusters identified. (D) Percentage of each cluster for each group. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Arrows in (C) and (D) indicate immune cell clusters of myeloid cells (blue), B cells (green) or T cells (red). " width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

    doi: 10.3389/fimmu.2026.1761769

    Figure Lengend Snippet: Multiparameter spectral flow cytometry analysis of immune cells of the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in Supplementary Figure 1 . (A) Automated T-distributed stochastic neighbour embedding (t-SNE) 2D map of the flow cytometry data acquired from control and infected mice colon. (B) Upper panel; tSNE 2D map showing scaled expression of CD11b for myeloid cells, B220 for B cells and CD3 for T cells. Lower panel; tSNE 2D map showing the location of CD11b + myeloid cells, B220 + B cells and CD3 + T cells. (C) Heat maps for (left) cell surface markers expression (CD45, CD11b, B220, CD3, CD4 and CD8) and (right) groups (control, acute and chronic colons) for 22 cell clusters identified. (D) Percentage of each cluster for each group. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Arrows in (C) and (D) indicate immune cell clusters of myeloid cells (blue), B cells (green) or T cells (red).

    Article Snippet: Antibodies were polyclonal goat IgG anti-mouse CD4 antibody (R&D Systems, MN), polyclonal rabbit IgG anti-mouse CD8 antibody (Novus Biologicals, CO), monoclonal Rat IgG2b Alexa Fluor ® 647 anti-mouse CD3 Antibody (BioLegend, CA), Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher Scientific, CA), Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 (Thermo Fisher Scientific, CA) ( ).

    Techniques: Flow Cytometry, Infection, Isolation, Control, Expressing, Comparison

    Phenotypic analysis of T cells via multiparameter spectral flow cytometry in the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in <xref ref-type=Supplementary Figure 1 . (A) T cells composition by flow cytometry, (B) tSNE 2D map showing scaled expression of CD3, CD4, and CD8 cell makers (C) Heat map of T cells (CD45 + , CD3 + ) (highlighted clusters 1, 7, 9, 16, 20, 22). Arrows indicate immune cell clusters of inflammatory (purple) and regulatory (pink) double-negative (DN) T cells. (D) tSNE 2D map of DN T cells and percentage of T cells cluster for each group. Arrows indicate DN T cell clusters of inflammatory (purple) and regulatory (pink) cells. (E) Percentage of DN T cells with inflammatory/regulatory phenotypes in total immune cells (CD45 + cells). (F) Relative percentage of DN T cells with inflammatory/regulatory phenotypes in total DN T cells. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Bars in A represent mean ± SD, and individual symbols denote values from single mice (n = 15; 5 per set, 3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, ****p < 0.0001. " width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

    doi: 10.3389/fimmu.2026.1761769

    Figure Lengend Snippet: Phenotypic analysis of T cells via multiparameter spectral flow cytometry in the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in Supplementary Figure 1 . (A) T cells composition by flow cytometry, (B) tSNE 2D map showing scaled expression of CD3, CD4, and CD8 cell makers (C) Heat map of T cells (CD45 + , CD3 + ) (highlighted clusters 1, 7, 9, 16, 20, 22). Arrows indicate immune cell clusters of inflammatory (purple) and regulatory (pink) double-negative (DN) T cells. (D) tSNE 2D map of DN T cells and percentage of T cells cluster for each group. Arrows indicate DN T cell clusters of inflammatory (purple) and regulatory (pink) cells. (E) Percentage of DN T cells with inflammatory/regulatory phenotypes in total immune cells (CD45 + cells). (F) Relative percentage of DN T cells with inflammatory/regulatory phenotypes in total DN T cells. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Bars in A represent mean ± SD, and individual symbols denote values from single mice (n = 15; 5 per set, 3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, ****p < 0.0001.

    Article Snippet: Antibodies were polyclonal goat IgG anti-mouse CD4 antibody (R&D Systems, MN), polyclonal rabbit IgG anti-mouse CD8 antibody (Novus Biologicals, CO), monoclonal Rat IgG2b Alexa Fluor ® 647 anti-mouse CD3 Antibody (BioLegend, CA), Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher Scientific, CA), Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 (Thermo Fisher Scientific, CA) ( ).

    Techniques: Flow Cytometry, Infection, Isolation, Control, Expressing, Comparison

    Double-negative T cells phenotypes in the colonic lamina propria of C57BL/6 mice during T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Mice were euthanized during the acute (30 dpi) and chronic (90 dpi) phases. Colonic lamina propria cells were isolated from uninfected (Control; blue), acutely infected (Acute; pink) and chronically infected (Chronic; green) mice. Cells were gated on single cells, live, CD45 + , CD3 + , CD4 - , CD8 - events and subsequently on (A) inflammatory immune cell markers including CCR5, CXCR3 or Granzyme B, and (B) regulatory immune cells markers including CCR4, IL10Rα or IL10. Bars represent mean ± SD, and individual symbols denote values from single mice (n = 8-12; 4 per set, 2–3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, **p < 0.01, ***p < 0.001, **** p ≤ 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

    doi: 10.3389/fimmu.2026.1761769

    Figure Lengend Snippet: Double-negative T cells phenotypes in the colonic lamina propria of C57BL/6 mice during T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Mice were euthanized during the acute (30 dpi) and chronic (90 dpi) phases. Colonic lamina propria cells were isolated from uninfected (Control; blue), acutely infected (Acute; pink) and chronically infected (Chronic; green) mice. Cells were gated on single cells, live, CD45 + , CD3 + , CD4 - , CD8 - events and subsequently on (A) inflammatory immune cell markers including CCR5, CXCR3 or Granzyme B, and (B) regulatory immune cells markers including CCR4, IL10Rα or IL10. Bars represent mean ± SD, and individual symbols denote values from single mice (n = 8-12; 4 per set, 2–3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, **p < 0.01, ***p < 0.001, **** p ≤ 0.0001.

    Article Snippet: Antibodies were polyclonal goat IgG anti-mouse CD4 antibody (R&D Systems, MN), polyclonal rabbit IgG anti-mouse CD8 antibody (Novus Biologicals, CO), monoclonal Rat IgG2b Alexa Fluor ® 647 anti-mouse CD3 Antibody (BioLegend, CA), Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher Scientific, CA), Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 (Thermo Fisher Scientific, CA) ( ).

    Techniques: Infection, Isolation, Control

    Microscopy analysis of T. cruzi infected colons. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc-RFP). Colons were isolated from control and chronically infected mice (90 dpi), processed for microscopy and stained with CD3 (Blue), CD4 (Green) and CD8 (Red) specific antibodies ( <xref ref-type=Supplementary Table 1 , Antibody Panel 4) as described in the materials and methods. Immunofluorescence images of cross sections of colons obtained from non-infected mice (Control) and chronically infected mice (Chronic) are shown in panels (A, B) , respectively. In (A, B) , a low magnification image of the entire colon is shown in the upper left, a region of interest (ROI) 1 is shown at mid-magnification in the lower left, and a high magnification image of ROI 2 is shown in shown on the right. For ROI 2, an overlay image of the 3 channels CD3 (Blue), CD4 (Green) and CD8 (Red) is shown on the top right, and the individual channels are represented below as indicated. A representative result of 3 controls and 3 infected colons is shown in this figure. LP, Lamina Propria; solid white arrow heads, double-negative T cells (CD3 + , CD4 - , CD8 - ); open arrowheads, CD3 - , CD4 - , CD8 + cells; solid pink arrowheads, CD3 - , CD4 + , CD8 - cells; solid yellow arrowheads, CD3 - , CD4 + , CD8 + cells. " width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

    doi: 10.3389/fimmu.2026.1761769

    Figure Lengend Snippet: Microscopy analysis of T. cruzi infected colons. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc-RFP). Colons were isolated from control and chronically infected mice (90 dpi), processed for microscopy and stained with CD3 (Blue), CD4 (Green) and CD8 (Red) specific antibodies ( Supplementary Table 1 , Antibody Panel 4) as described in the materials and methods. Immunofluorescence images of cross sections of colons obtained from non-infected mice (Control) and chronically infected mice (Chronic) are shown in panels (A, B) , respectively. In (A, B) , a low magnification image of the entire colon is shown in the upper left, a region of interest (ROI) 1 is shown at mid-magnification in the lower left, and a high magnification image of ROI 2 is shown in shown on the right. For ROI 2, an overlay image of the 3 channels CD3 (Blue), CD4 (Green) and CD8 (Red) is shown on the top right, and the individual channels are represented below as indicated. A representative result of 3 controls and 3 infected colons is shown in this figure. LP, Lamina Propria; solid white arrow heads, double-negative T cells (CD3 + , CD4 - , CD8 - ); open arrowheads, CD3 - , CD4 - , CD8 + cells; solid pink arrowheads, CD3 - , CD4 + , CD8 - cells; solid yellow arrowheads, CD3 - , CD4 + , CD8 + cells.

    Article Snippet: Antibodies were polyclonal goat IgG anti-mouse CD4 antibody (R&D Systems, MN), polyclonal rabbit IgG anti-mouse CD8 antibody (Novus Biologicals, CO), monoclonal Rat IgG2b Alexa Fluor ® 647 anti-mouse CD3 Antibody (BioLegend, CA), Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher Scientific, CA), Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 (Thermo Fisher Scientific, CA) ( ).

    Techniques: Microscopy, Infection, Isolation, Control, Staining, Immunofluorescence

    Cellular and gene expression analyses of stem cell activation elicited by sequential running stimuli in p16 KO and p16 WT dentate gyrus. (A) Timeline of the experiments performed on p16 WT and p16 KO 1-year-old mice: i) no run; ii) single run of 7 days; iii) single run of 12 days followed by a sedentary period; iv) double run of 12 and 7 days spaced 21 days apart. (B) Representative images by confocal microscopy (×40 magnification) showing that the proliferating type-1 stem cells (Ki67 + /Sox2 + /GFAP + , labeled in red, blue and green, respectively) in the dentate gyrus of p16 KO mice increase, relative to p16 WT, after a single run of 12 days followed by a sedentary period, whereas they do not increase after a double run of 12 and 7 days spaced 21 days apart. The white dashed line labels the boundaries of the dentate gyrus. Arrowheads: Ki67 + /Sox2 + /GFAP − cells, arrows: Ki67 + /Sox2 + /GFAP + cells. Scale bar 50 μm. White boxes: area at higher magnification (×2), shown in the panels on the right. (C) Graph showing the changes induced by running on the proliferation of stem cells (type-1 cells, Ki67 + /Sox2 + /GFAP + ) in p16 WT and in p16 KO dentate gyrus, in the different groups indicated. A single RUN of 12 days induces the proliferation of p16 KO stem cells for an extended period (28 days; group 12d RUN +28d), but a second running added (7 days; group 12d RUN +21d + 7d RUN) does not stimulate p16 KO stem cell proliferation. (D) Similar changes were observed analyzing stem and progenitor cells together (i.e., type-1 and type-2a cells, respectively; Ki67 + /Sox2 + cells). (C,D) Analysis of simple effects: NS p > 0.05, ** p < 0.01, or **** p < 0.0001, PLSD ANOVA test. Cell numbers in the dentate gyrus are means ± SEM of the analysis of five animals per group. (E) Real-time PCR analysis of the differential expression response to the second running stimulus for the Set A_Set E_GLM genes whose activation by running had been validated, i.e., Tfap2c , Lepr , Top2a , Slc18a2 , Lpin2 , Rgs14 , Ramp3 , Chrm5 . The expression of these genes was activated in the p16 knockout dentate gyrus by a single run of 12 days but was not changed by a second running, relative to p16 wild-type. The figure shows the mean mRNA expression fold increases ±SEM from two independent experiments. Four biological replicates were used for mice that run 12 days and five biological replicates for mice that underwent the double run. TBP was used to normalize data. The statistical analysis of real-time PCR data was performed by non-parametric Kruskal-Wallis test to detect main effects (resulting with p -value <0.05 for all the genes tested), followed by Mann-Whitney U post hoc test for single Set comparison. * p < 0.05, ** p < 0.01, *** p < 0.001, Mann-Whitney U post hoc test; n = at least 24, i.e., total number of data for each gene analyzed for all four groups (WT RUN and KO RUN, WT 12d RUN +21d + 7d RUN or KO 12d RUN +21d + 7d RUN).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Transcriptome analysis reveals genes associated with stem cell activation by physical exercise in the dentate gyrus of aged p16Ink4a knockout mice

    doi: 10.3389/fcell.2023.1270892

    Figure Lengend Snippet: Cellular and gene expression analyses of stem cell activation elicited by sequential running stimuli in p16 KO and p16 WT dentate gyrus. (A) Timeline of the experiments performed on p16 WT and p16 KO 1-year-old mice: i) no run; ii) single run of 7 days; iii) single run of 12 days followed by a sedentary period; iv) double run of 12 and 7 days spaced 21 days apart. (B) Representative images by confocal microscopy (×40 magnification) showing that the proliferating type-1 stem cells (Ki67 + /Sox2 + /GFAP + , labeled in red, blue and green, respectively) in the dentate gyrus of p16 KO mice increase, relative to p16 WT, after a single run of 12 days followed by a sedentary period, whereas they do not increase after a double run of 12 and 7 days spaced 21 days apart. The white dashed line labels the boundaries of the dentate gyrus. Arrowheads: Ki67 + /Sox2 + /GFAP − cells, arrows: Ki67 + /Sox2 + /GFAP + cells. Scale bar 50 μm. White boxes: area at higher magnification (×2), shown in the panels on the right. (C) Graph showing the changes induced by running on the proliferation of stem cells (type-1 cells, Ki67 + /Sox2 + /GFAP + ) in p16 WT and in p16 KO dentate gyrus, in the different groups indicated. A single RUN of 12 days induces the proliferation of p16 KO stem cells for an extended period (28 days; group 12d RUN +28d), but a second running added (7 days; group 12d RUN +21d + 7d RUN) does not stimulate p16 KO stem cell proliferation. (D) Similar changes were observed analyzing stem and progenitor cells together (i.e., type-1 and type-2a cells, respectively; Ki67 + /Sox2 + cells). (C,D) Analysis of simple effects: NS p > 0.05, ** p < 0.01, or **** p < 0.0001, PLSD ANOVA test. Cell numbers in the dentate gyrus are means ± SEM of the analysis of five animals per group. (E) Real-time PCR analysis of the differential expression response to the second running stimulus for the Set A_Set E_GLM genes whose activation by running had been validated, i.e., Tfap2c , Lepr , Top2a , Slc18a2 , Lpin2 , Rgs14 , Ramp3 , Chrm5 . The expression of these genes was activated in the p16 knockout dentate gyrus by a single run of 12 days but was not changed by a second running, relative to p16 wild-type. The figure shows the mean mRNA expression fold increases ±SEM from two independent experiments. Four biological replicates were used for mice that run 12 days and five biological replicates for mice that underwent the double run. TBP was used to normalize data. The statistical analysis of real-time PCR data was performed by non-parametric Kruskal-Wallis test to detect main effects (resulting with p -value <0.05 for all the genes tested), followed by Mann-Whitney U post hoc test for single Set comparison. * p < 0.05, ** p < 0.01, *** p < 0.001, Mann-Whitney U post hoc test; n = at least 24, i.e., total number of data for each gene analyzed for all four groups (WT RUN and KO RUN, WT 12d RUN +21d + 7d RUN or KO 12d RUN +21d + 7d RUN).

    Article Snippet: Proliferating stem and progenitor cells were visualized by means of a rabbit monoclonal antibody against Ki67 (Invitrogen, San Diego, CA, USA; MA514520; 1:200), a goat polyclonal antibody against Sox2 (Abcam, Cambridge, UK; Ab239218; 1:300), and a mouse monoclonal antibody against GFAP (Sigma-Aldrich, St Louis, MO; G6171; 1:200).

    Techniques: Expressing, Activation Assay, Confocal Microscopy, Labeling, Real-time Polymerase Chain Reaction, Knock-Out, MANN-WHITNEY

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Transcriptome analysis reveals genes associated with stem cell activation by physical exercise in the dentate gyrus of aged p16Ink4a knockout mice

    doi: 10.3389/fcell.2023.1270892

    Figure Lengend Snippet:

    Article Snippet: Proliferating stem and progenitor cells were visualized by means of a rabbit monoclonal antibody against Ki67 (Invitrogen, San Diego, CA, USA; MA514520; 1:200), a goat polyclonal antibody against Sox2 (Abcam, Cambridge, UK; Ab239218; 1:300), and a mouse monoclonal antibody against GFAP (Sigma-Aldrich, St Louis, MO; G6171; 1:200).

    Techniques: Translocation Assay, Real-time Polymerase Chain Reaction, Knock-Out, Activity Assay

    Inclusion and exclusion criteria.

    Journal: Biology

    Article Title: Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema

    doi: 10.3390/biology10090934

    Figure Lengend Snippet: Inclusion and exclusion criteria.

    Article Snippet: Primary antibodies for immunohistochemical staining included rabbit polyclonal anti-human collagen I (ab34170; Abcam, Cambridge, UK), rabbit polyclonal anti-human Ki67 (ab15580; Abcam), goat polyclonal anti-human CD4 (AF379; R&D Systems, Minneapolis, MN, USA), goat polyclonal anti-human LYVE-1 (AF2089; R&D Systems), rabbit polyclonal anti-human periostin (ab14041; Abcam), rabbit polyclonal anti-human IL13 (ab9576; Abcam), mouse monoclonal anti-human mast cell tryptase (ab2378; Abcam), rabbit polyclonal anti-human thymic stromal lymphopoietin (TSLP; ab188766; Abcam), guinea pig polyclonal anti-human cytokeratin14 (ab192694; Abcam), goat polyclonal anti-human IL33 (AF3625; R&D System), rat monoclonal anti-IL25 (NBP2-11677; Novus Biologicals, Centennial, Colorado), rabbit polyclonal anti-human IL13 receptor (ab79277; Abcam), mouse monoclonal anti-human IL4 (MAB304; R&D Systems), and mouse monoclonal anti-human IL5 (MAB605; R&D Systems).

    Techniques: Dissection, Bioprocessing

    Treatment with QBX258 decreases mast cell infiltration in lymphedematous skin. ( a ) Representative low-power (upper images) and high-power (lower images) immunofluorescent staining (left panels) and quantification (right panel) of DAPI (blue) and CD4 + cells (pink) in normal, LE, and LE + tx biopsy specimens. Area in dotted box is shown in high-power views. ( b ) Representative low-power (upper images) and high-power (lower images) immunofluorescent staining (left panels) and quantification (right panel) of DAPI (blue), LYVE-1 (red), and tryptase (green) in normal, LE, and LE + tx biopsy specimens. Area in dotted box is shown in high-power views. Scale bar (low-power images): 200 μm; scale bar (magnified images): 25 μm; * p < 0.05; ** p < 0.01.

    Journal: Biology

    Article Title: Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema

    doi: 10.3390/biology10090934

    Figure Lengend Snippet: Treatment with QBX258 decreases mast cell infiltration in lymphedematous skin. ( a ) Representative low-power (upper images) and high-power (lower images) immunofluorescent staining (left panels) and quantification (right panel) of DAPI (blue) and CD4 + cells (pink) in normal, LE, and LE + tx biopsy specimens. Area in dotted box is shown in high-power views. ( b ) Representative low-power (upper images) and high-power (lower images) immunofluorescent staining (left panels) and quantification (right panel) of DAPI (blue), LYVE-1 (red), and tryptase (green) in normal, LE, and LE + tx biopsy specimens. Area in dotted box is shown in high-power views. Scale bar (low-power images): 200 μm; scale bar (magnified images): 25 μm; * p < 0.05; ** p < 0.01.

    Article Snippet: Primary antibodies for immunohistochemical staining included rabbit polyclonal anti-human collagen I (ab34170; Abcam, Cambridge, UK), rabbit polyclonal anti-human Ki67 (ab15580; Abcam), goat polyclonal anti-human CD4 (AF379; R&D Systems, Minneapolis, MN, USA), goat polyclonal anti-human LYVE-1 (AF2089; R&D Systems), rabbit polyclonal anti-human periostin (ab14041; Abcam), rabbit polyclonal anti-human IL13 (ab9576; Abcam), mouse monoclonal anti-human mast cell tryptase (ab2378; Abcam), rabbit polyclonal anti-human thymic stromal lymphopoietin (TSLP; ab188766; Abcam), guinea pig polyclonal anti-human cytokeratin14 (ab192694; Abcam), goat polyclonal anti-human IL33 (AF3625; R&D System), rat monoclonal anti-IL25 (NBP2-11677; Novus Biologicals, Centennial, Colorado), rabbit polyclonal anti-human IL13 receptor (ab79277; Abcam), mouse monoclonal anti-human IL4 (MAB304; R&D Systems), and mouse monoclonal anti-human IL5 (MAB605; R&D Systems).

    Techniques: Staining