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polyclonal goat igg anti mouse cd4 antibody (R&D Systems)

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R&D Systems polyclonal goat igg anti mouse cd4 antibody

Multiparameter spectral flow cytometry analysis of immune cells of the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in <xref ref-type=

polyclonal goat igg anti mouse cd4 antibody - by Bioz Stars, 2026-09

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1) Product Images from "Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease"

Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2026.1761769

Supplementary Figure 1 . (A) Automated T-distributed stochastic neighbour embedding (t-SNE) 2D map of the flow cytometry data acquired from control and infected mice colon. (B) Upper panel; tSNE 2D map showing scaled expression of CD11b for myeloid cells, B220 for B cells and CD3 for T cells. Lower panel; tSNE 2D map showing the location of CD11b + myeloid cells, B220 + B cells and CD3 + T cells. (C) Heat maps for (left) cell surface markers expression (CD45, CD11b, B220, CD3, CD4 and CD8) and (right) groups (control, acute and chronic colons) for 22 cell clusters identified. (D) Percentage of each cluster for each group. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Arrows in (C) and (D) indicate immune cell clusters of myeloid cells (blue), B cells (green) or T cells (red). " title="... cell surface markers expression (CD45, CD11b, B220, CD3, CD4 and CD8) and (right) groups (control, acute and ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: Multiparameter spectral flow cytometry analysis of immune cells of the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in Supplementary Figure 1 . (A) Automated T-distributed stochastic neighbour embedding (t-SNE) 2D map of the flow cytometry data acquired from control and infected mice colon. (B) Upper panel; tSNE 2D map showing scaled expression of CD11b for myeloid cells, B220 for B cells and CD3 for T cells. Lower panel; tSNE 2D map showing the location of CD11b + myeloid cells, B220 + B cells and CD3 + T cells. (C) Heat maps for (left) cell surface markers expression (CD45, CD11b, B220, CD3, CD4 and CD8) and (right) groups (control, acute and chronic colons) for 22 cell clusters identified. (D) Percentage of each cluster for each group. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Arrows in (C) and (D) indicate immune cell clusters of myeloid cells (blue), B cells (green) or T cells (red).

Techniques Used: Flow Cytometry, Infection, Isolation, Control, Expressing, Comparison

Phenotypic analysis of T cells via multiparameter spectral flow cytometry in the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in <xref ref-type=

Supplementary Figure 1 . (A) T cells composition by flow cytometry, (B) tSNE 2D map showing scaled expression of CD3, CD4, and CD8 cell makers (C) Heat map of T cells (CD45 + , CD3 + ) (highlighted clusters 1, 7, 9, 16, 20, 22). Arrows indicate immune cell clusters of inflammatory (purple) and regulatory (pink) double-negative (DN) T cells. (D) tSNE 2D map of DN T cells and percentage of T cells cluster for each group. Arrows indicate DN T cell clusters of inflammatory (purple) and regulatory (pink) cells. (E) Percentage of DN T cells with inflammatory/regulatory phenotypes in total immune cells (CD45 + cells). (F) Relative percentage of DN T cells with inflammatory/regulatory phenotypes in total DN T cells. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Bars in A represent mean ± SD, and individual symbols denote values from single mice (n = 15; 5 per set, 3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, ****p < 0.0001. " title="... tSNE 2D map showing scaled expression of CD3, CD4, and CD8 cell makers (C) Heat map of ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: Phenotypic analysis of T cells via multiparameter spectral flow cytometry in the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in Supplementary Figure 1 . (A) T cells composition by flow cytometry, (B) tSNE 2D map showing scaled expression of CD3, CD4, and CD8 cell makers (C) Heat map of T cells (CD45 + , CD3 + ) (highlighted clusters 1, 7, 9, 16, 20, 22). Arrows indicate immune cell clusters of inflammatory (purple) and regulatory (pink) double-negative (DN) T cells. (D) tSNE 2D map of DN T cells and percentage of T cells cluster for each group. Arrows indicate DN T cell clusters of inflammatory (purple) and regulatory (pink) cells. (E) Percentage of DN T cells with inflammatory/regulatory phenotypes in total immune cells (CD45 + cells). (F) Relative percentage of DN T cells with inflammatory/regulatory phenotypes in total DN T cells. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Bars in A represent mean ± SD, and individual symbols denote values from single mice (n = 15; 5 per set, 3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, ****p < 0.0001.

Techniques Used: Flow Cytometry, Infection, Isolation, Control, Expressing, Comparison

Double-negative T cells phenotypes in the colonic lamina propria of C57BL/6 mice during T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Mice were euthanized during the acute (30 dpi) and chronic (90 dpi) phases. Colonic lamina propria cells were isolated from uninfected (Control; blue), acutely infected (Acute; pink) and chronically infected (Chronic; green) mice. Cells were gated on single cells, live, CD45 + , CD3 + , CD4 - , CD8 - events and subsequently on (A) inflammatory immune cell markers including CCR5, CXCR3 or Granzyme B, and (B) regulatory immune cells markers including CCR4, IL10Rα or IL10. Bars represent mean ± SD, and individual symbols denote values from single mice (n = 8-12; 4 per set, 2–3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, **p < 0.01, ***p < 0.001, **** p ≤ 0.0001.

Figure Legend Snippet: Double-negative T cells phenotypes in the colonic lamina propria of C57BL/6 mice during T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Mice were euthanized during the acute (30 dpi) and chronic (90 dpi) phases. Colonic lamina propria cells were isolated from uninfected (Control; blue), acutely infected (Acute; pink) and chronically infected (Chronic; green) mice. Cells were gated on single cells, live, CD45 + , CD3 + , CD4 - , CD8 - events and subsequently on (A) inflammatory immune cell markers including CCR5, CXCR3 or Granzyme B, and (B) regulatory immune cells markers including CCR4, IL10Rα or IL10. Bars represent mean ± SD, and individual symbols denote values from single mice (n = 8-12; 4 per set, 2–3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, **p < 0.01, ***p < 0.001, **** p ≤ 0.0001.

Techniques Used: Infection, Isolation, Control

Microscopy analysis of T. cruzi infected colons. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc-RFP). Colons were isolated from control and chronically infected mice (90 dpi), processed for microscopy and stained with CD3 (Blue), CD4 (Green) and CD8 (Red) specific antibodies ( <xref ref-type=

Supplementary Table 1 , Antibody Panel 4) as described in the materials and methods. Immunofluorescence images of cross sections of colons obtained from non-infected mice (Control) and chronically infected mice (Chronic) are shown in panels (A, B) , respectively. In (A, B) , a low magnification image of the entire colon is shown in the upper left, a region of interest (ROI) 1 is shown at mid-magnification in the lower left, and a high magnification image of ROI 2 is shown in shown on the right. For ROI 2, an overlay image of the 3 channels CD3 (Blue), CD4 (Green) and CD8 (Red) is shown on the top right, and the individual channels are represented below as indicated. A representative result of 3 controls and 3 infected colons is shown in this figure. LP, Lamina Propria; solid white arrow heads, double-negative T cells (CD3 + , CD4 - , CD8 - ); open arrowheads, CD3 - , CD4 - , CD8 + cells; solid pink arrowheads, CD3 - , CD4 + , CD8 - cells; solid yellow arrowheads, CD3 - , CD4 + , CD8 + cells. " title="... processed for microscopy and stained with CD3 (Blue), CD4 (Green) and CD8 (Red) specific antibodies (
Figure Legend Snippet: Microscopy analysis of T. cruzi infected colons. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc-RFP). Colons were isolated from control and chronically infected mice (90 dpi), processed for microscopy and stained with CD3 (Blue), CD4 (Green) and CD8 (Red) specific antibodies ( Supplementary Table 1 , Antibody Panel 4) as described in the materials and methods. Immunofluorescence images of cross sections of colons obtained from non-infected mice (Control) and chronically infected mice (Chronic) are shown in panels (A, B) , respectively. In (A, B) , a low magnification image of the entire colon is shown in the upper left, a region of interest (ROI) 1 is shown at mid-magnification in the lower left, and a high magnification image of ROI 2 is shown in shown on the right. For ROI 2, an overlay image of the 3 channels CD3 (Blue), CD4 (Green) and CD8 (Red) is shown on the top right, and the individual channels are represented below as indicated. A representative result of 3 controls and 3 infected colons is shown in this figure. LP, Lamina Propria; solid white arrow heads, double-negative T cells (CD3 + , CD4 - , CD8 - ); open arrowheads, CD3 - , CD4 - , CD8 + cells; solid pink arrowheads, CD3 - , CD4 + , CD8 - cells; solid yellow arrowheads, CD3 - , CD4 + , CD8 + cells.

Techniques Used: Microscopy, Infection, Isolation, Control, Staining, Immunofluorescence

Incubation:

Article Title: AZD5582 plus SIV-specific antibodies reduce lymph node viral reservoirs in antiretroviral therapy-suppressed macaques.
Article Snippet: .. In brief, after heat-induced epitope retrieval (HIER) (Advanced Cell Diagnostics), antibodies were incubated overnight at room temperature: rabbit polyclonal anti-CD8 (1:1,000, HNBP2-34039, Novus), mouse monoclonal anti-CD20 (1:500, M0755, DAKO) and goat polyclonal anti-CD4 (1:1,000, AF-379-NA, R&D Systems). .. Slides were washed and then incubated with secondary donkey anti-goat IgG-Alexa Fluor 488, donkey anti-mouse IgG-Alexa Fluor 594 and anti-rabbit IgG-Alexa Fluor 647 (all from Molecular Probes/Thermo Fisher Scientific) for 1 h at room temperature and washed three times for 5 min in TBS + Tween (0.05% v/v).

Article Title: AZD5582 plus SIV-specific antibodies reduce lymph node viral reservoirs in antiretroviral therapy-suppressed macaques
Article Snippet: .. In brief, after heat-induced epitope retrieval (HIER) (Advanced Cell Diagnostics), antibodies were incubated overnight at room temperature: rabbit polyclonal anti-CD8 (1:1,000, HNBP2-34039, Novus), mouse monoclonal anti-CD20 (1:500, M0755, DAKO) and goat polyclonal anti-CD4 (1:1,000, AF-379-NA, R&D Systems). .. Slides were washed and then incubated with secondary donkey anti-goat IgG-Alexa Fluor 488, donkey anti-mouse IgG-Alexa Fluor 594 and anti-rabbit IgG-Alexa Fluor 647 (all from Molecular Probes/Thermo Fisher Scientific) for 1 h at room temperature and washed three times for 5 min in TBS + Tween (0.05% v/v).

other:

Article Title: A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry
Article Snippet: Goat polyclonal anti-CD4 , R and D Systems , CatAF-379-NA, RRID:AB_354469.

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Journal: Frontiers in Immunology

Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

doi: 10.3389/fimmu.2026.1761769

Figure Lengend Snippet: Multiparameter spectral flow cytometry analysis of immune cells of the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in Supplementary Figure 1 . (A) Automated T-distributed stochastic neighbour embedding (t-SNE) 2D map of the flow cytometry data acquired from control and infected mice colon. (B) Upper panel; tSNE 2D map showing scaled expression of CD11b for myeloid cells, B220 for B cells and CD3 for T cells. Lower panel; tSNE 2D map showing the location of CD11b + myeloid cells, B220 + B cells and CD3 + T cells. (C) Heat maps for (left) cell surface markers expression (CD45, CD11b, B220, CD3, CD4 and CD8) and (right) groups (control, acute and chronic colons) for 22 cell clusters identified. (D) Percentage of each cluster for each group. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Arrows in (C) and (D) indicate immune cell clusters of myeloid cells (blue), B cells (green) or T cells (red).

Article Snippet: Antibodies were polyclonal goat IgG anti-mouse CD4 antibody (R&D Systems, MN), polyclonal rabbit IgG anti-mouse CD8 antibody (Novus Biologicals, CO), monoclonal Rat IgG2b Alexa Fluor ® 647 anti-mouse CD3 Antibody (BioLegend, CA), Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher Scientific, CA), Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 (Thermo Fisher Scientific, CA) ( ).

Techniques: Flow Cytometry, Infection, Isolation, Control, Expressing, Comparison

Journal: Frontiers in Immunology

Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

doi: 10.3389/fimmu.2026.1761769

Figure Lengend Snippet: Phenotypic analysis of T cells via multiparameter spectral flow cytometry in the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in Supplementary Figure 1 . (A) T cells composition by flow cytometry, (B) tSNE 2D map showing scaled expression of CD3, CD4, and CD8 cell makers (C) Heat map of T cells (CD45 + , CD3 + ) (highlighted clusters 1, 7, 9, 16, 20, 22). Arrows indicate immune cell clusters of inflammatory (purple) and regulatory (pink) double-negative (DN) T cells. (D) tSNE 2D map of DN T cells and percentage of T cells cluster for each group. Arrows indicate DN T cell clusters of inflammatory (purple) and regulatory (pink) cells. (E) Percentage of DN T cells with inflammatory/regulatory phenotypes in total immune cells (CD45 + cells). (F) Relative percentage of DN T cells with inflammatory/regulatory phenotypes in total DN T cells. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Bars in A represent mean ± SD, and individual symbols denote values from single mice (n = 15; 5 per set, 3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, ****p < 0.0001.

Techniques: Flow Cytometry, Infection, Isolation, Control, Expressing, Comparison

Journal: Frontiers in Immunology

Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

doi: 10.3389/fimmu.2026.1761769

Figure Lengend Snippet: Double-negative T cells phenotypes in the colonic lamina propria of C57BL/6 mice during T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Mice were euthanized during the acute (30 dpi) and chronic (90 dpi) phases. Colonic lamina propria cells were isolated from uninfected (Control; blue), acutely infected (Acute; pink) and chronically infected (Chronic; green) mice. Cells were gated on single cells, live, CD45 + , CD3 + , CD4 - , CD8 - events and subsequently on (A) inflammatory immune cell markers including CCR5, CXCR3 or Granzyme B, and (B) regulatory immune cells markers including CCR4, IL10Rα or IL10. Bars represent mean ± SD, and individual symbols denote values from single mice (n = 8-12; 4 per set, 2–3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, **p < 0.01, ***p < 0.001, **** p ≤ 0.0001.

Techniques: Infection, Isolation, Control

Journal: Frontiers in Immunology

Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

doi: 10.3389/fimmu.2026.1761769

Figure Lengend Snippet: Microscopy analysis of T. cruzi infected colons. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc-RFP). Colons were isolated from control and chronically infected mice (90 dpi), processed for microscopy and stained with CD3 (Blue), CD4 (Green) and CD8 (Red) specific antibodies ( Supplementary Table 1 , Antibody Panel 4) as described in the materials and methods. Immunofluorescence images of cross sections of colons obtained from non-infected mice (Control) and chronically infected mice (Chronic) are shown in panels (A, B) , respectively. In (A, B) , a low magnification image of the entire colon is shown in the upper left, a region of interest (ROI) 1 is shown at mid-magnification in the lower left, and a high magnification image of ROI 2 is shown in shown on the right. For ROI 2, an overlay image of the 3 channels CD3 (Blue), CD4 (Green) and CD8 (Red) is shown on the top right, and the individual channels are represented below as indicated. A representative result of 3 controls and 3 infected colons is shown in this figure. LP, Lamina Propria; solid white arrow heads, double-negative T cells (CD3 + , CD4 - , CD8 - ); open arrowheads, CD3 - , CD4 - , CD8 + cells; solid pink arrowheads, CD3 - , CD4 + , CD8 - cells; solid yellow arrowheads, CD3 - , CD4 + , CD8 + cells.

Techniques: Microscopy, Infection, Isolation, Control, Staining, Immunofluorescence

Cellular and gene expression analyses of stem cell activation elicited by sequential running stimuli in p16 KO and p16 WT dentate gyrus. (A) Timeline of the experiments performed on p16 WT and p16 KO 1-year-old mice: i) no run; ii) single run of 7 days; iii) single run of 12 days followed by a sedentary period; iv) double run of 12 and 7 days spaced 21 days apart. (B) Representative images by confocal microscopy (×40 magnification) showing that the proliferating type-1 stem cells (Ki67 + /Sox2 + /GFAP + , labeled in red, blue and green, respectively) in the dentate gyrus of p16 KO mice increase, relative to p16 WT, after a single run of 12 days followed by a sedentary period, whereas they do not increase after a double run of 12 and 7 days spaced 21 days apart. The white dashed line labels the boundaries of the dentate gyrus. Arrowheads: Ki67 + /Sox2 + /GFAP − cells, arrows: Ki67 + /Sox2 + /GFAP + cells. Scale bar 50 μm. White boxes: area at higher magnification (×2), shown in the panels on the right. (C) Graph showing the changes induced by running on the proliferation of stem cells (type-1 cells, Ki67 + /Sox2 + /GFAP + ) in p16 WT and in p16 KO dentate gyrus, in the different groups indicated. A single RUN of 12 days induces the proliferation of p16 KO stem cells for an extended period (28 days; group 12d RUN +28d), but a second running added (7 days; group 12d RUN +21d + 7d RUN) does not stimulate p16 KO stem cell proliferation. (D) Similar changes were observed analyzing stem and progenitor cells together (i.e., type-1 and type-2a cells, respectively; Ki67 + /Sox2 + cells). (C,D) Analysis of simple effects: NS p > 0.05, ** p < 0.01, or **** p < 0.0001, PLSD ANOVA test. Cell numbers in the dentate gyrus are means ± SEM of the analysis of five animals per group. (E) Real-time PCR analysis of the differential expression response to the second running stimulus for the Set A_Set E_GLM genes whose activation by running had been validated, i.e., Tfap2c , Lepr , Top2a , Slc18a2 , Lpin2 , Rgs14 , Ramp3 , Chrm5 . The expression of these genes was activated in the p16 knockout dentate gyrus by a single run of 12 days but was not changed by a second running, relative to p16 wild-type. The figure shows the mean mRNA expression fold increases ±SEM from two independent experiments. Four biological replicates were used for mice that run 12 days and five biological replicates for mice that underwent the double run. TBP was used to normalize data. The statistical analysis of real-time PCR data was performed by non-parametric Kruskal-Wallis test to detect main effects (resulting with p -value <0.05 for all the genes tested), followed by Mann-Whitney U post hoc test for single Set comparison. * p < 0.05, ** p < 0.01, *** p < 0.001, Mann-Whitney U post hoc test; n = at least 24, i.e., total number of data for each gene analyzed for all four groups (WT RUN and KO RUN, WT 12d RUN +21d + 7d RUN or KO 12d RUN +21d + 7d RUN).

Journal: Frontiers in Cell and Developmental Biology

Article Title: Transcriptome analysis reveals genes associated with stem cell activation by physical exercise in the dentate gyrus of aged p16Ink4a knockout mice

doi: 10.3389/fcell.2023.1270892

Figure Lengend Snippet: Cellular and gene expression analyses of stem cell activation elicited by sequential running stimuli in p16 KO and p16 WT dentate gyrus. (A) Timeline of the experiments performed on p16 WT and p16 KO 1-year-old mice: i) no run; ii) single run of 7 days; iii) single run of 12 days followed by a sedentary period; iv) double run of 12 and 7 days spaced 21 days apart. (B) Representative images by confocal microscopy (×40 magnification) showing that the proliferating type-1 stem cells (Ki67 + /Sox2 + /GFAP + , labeled in red, blue and green, respectively) in the dentate gyrus of p16 KO mice increase, relative to p16 WT, after a single run of 12 days followed by a sedentary period, whereas they do not increase after a double run of 12 and 7 days spaced 21 days apart. The white dashed line labels the boundaries of the dentate gyrus. Arrowheads: Ki67 + /Sox2 + /GFAP − cells, arrows: Ki67 + /Sox2 + /GFAP + cells. Scale bar 50 μm. White boxes: area at higher magnification (×2), shown in the panels on the right. (C) Graph showing the changes induced by running on the proliferation of stem cells (type-1 cells, Ki67 + /Sox2 + /GFAP + ) in p16 WT and in p16 KO dentate gyrus, in the different groups indicated. A single RUN of 12 days induces the proliferation of p16 KO stem cells for an extended period (28 days; group 12d RUN +28d), but a second running added (7 days; group 12d RUN +21d + 7d RUN) does not stimulate p16 KO stem cell proliferation. (D) Similar changes were observed analyzing stem and progenitor cells together (i.e., type-1 and type-2a cells, respectively; Ki67 + /Sox2 + cells). (C,D) Analysis of simple effects: NS p > 0.05, ** p < 0.01, or **** p < 0.0001, PLSD ANOVA test. Cell numbers in the dentate gyrus are means ± SEM of the analysis of five animals per group. (E) Real-time PCR analysis of the differential expression response to the second running stimulus for the Set A_Set E_GLM genes whose activation by running had been validated, i.e., Tfap2c , Lepr , Top2a , Slc18a2 , Lpin2 , Rgs14 , Ramp3 , Chrm5 . The expression of these genes was activated in the p16 knockout dentate gyrus by a single run of 12 days but was not changed by a second running, relative to p16 wild-type. The figure shows the mean mRNA expression fold increases ±SEM from two independent experiments. Four biological replicates were used for mice that run 12 days and five biological replicates for mice that underwent the double run. TBP was used to normalize data. The statistical analysis of real-time PCR data was performed by non-parametric Kruskal-Wallis test to detect main effects (resulting with p -value <0.05 for all the genes tested), followed by Mann-Whitney U post hoc test for single Set comparison. * p < 0.05, ** p < 0.01, *** p < 0.001, Mann-Whitney U post hoc test; n = at least 24, i.e., total number of data for each gene analyzed for all four groups (WT RUN and KO RUN, WT 12d RUN +21d + 7d RUN or KO 12d RUN +21d + 7d RUN).

Article Snippet: Proliferating stem and progenitor cells were visualized by means of a rabbit monoclonal antibody against Ki67 (Invitrogen, San Diego, CA, USA; MA514520; 1:200), a goat polyclonal antibody against Sox2 (Abcam, Cambridge, UK; Ab239218; 1:300), and a mouse monoclonal antibody against GFAP (Sigma-Aldrich, St Louis, MO; G6171; 1:200).

Techniques: Gene Expression, Activation Assay, Confocal Microscopy, Labeling, Real-time Polymerase Chain Reaction, Quantitative Proteomics, Expressing, Knock-Out, MANN-WHITNEY, Comparison

Journal: Frontiers in Cell and Developmental Biology

Article Title: Transcriptome analysis reveals genes associated with stem cell activation by physical exercise in the dentate gyrus of aged p16Ink4a knockout mice

doi: 10.3389/fcell.2023.1270892

Figure Lengend Snippet:

Techniques: Translocation Assay, Real-time Polymerase Chain Reaction, Knock-Out, Saline, Activity Assay

Journal: Biology

Article Title: Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema

doi: 10.3390/biology10090934

Figure Lengend Snippet: Inclusion and exclusion criteria.

Article Snippet: Primary antibodies for immunohistochemical staining included rabbit polyclonal anti-human collagen I (ab34170; Abcam, Cambridge, UK), rabbit polyclonal anti-human Ki67 (ab15580; Abcam), goat polyclonal anti-human CD4 (AF379; R&D Systems, Minneapolis, MN, USA), goat polyclonal anti-human LYVE-1 (AF2089; R&D Systems), rabbit polyclonal anti-human periostin (ab14041; Abcam), rabbit polyclonal anti-human IL13 (ab9576; Abcam), mouse monoclonal anti-human mast cell tryptase (ab2378; Abcam), rabbit polyclonal anti-human thymic stromal lymphopoietin (TSLP; ab188766; Abcam), guinea pig polyclonal anti-human cytokeratin14 (ab192694; Abcam), goat polyclonal anti-human IL33 (AF3625; R&D System), rat monoclonal anti-IL25 (NBP2-11677; Novus Biologicals, Centennial, Colorado), rabbit polyclonal anti-human IL13 receptor (ab79277; Abcam), mouse monoclonal anti-human IL4 (MAB304; R&D Systems), and mouse monoclonal anti-human IL5 (MAB605; R&D Systems).

Techniques: Dissection, Bioprocessing

Treatment with QBX258 decreases mast cell infiltration in lymphedematous skin. ( a ) Representative low-power (upper images) and high-power (lower images) immunofluorescent staining (left panels) and quantification (right panel) of DAPI (blue) and CD4 + cells (pink) in normal, LE, and LE + tx biopsy specimens. Area in dotted box is shown in high-power views. ( b ) Representative low-power (upper images) and high-power (lower images) immunofluorescent staining (left panels) and quantification (right panel) of DAPI (blue), LYVE-1 (red), and tryptase (green) in normal, LE, and LE + tx biopsy specimens. Area in dotted box is shown in high-power views. Scale bar (low-power images): 200 μm; scale bar (magnified images): 25 μm; * p < 0.05; ** p < 0.01.

Journal: Biology

Article Title: Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema

doi: 10.3390/biology10090934

Figure Lengend Snippet: Treatment with QBX258 decreases mast cell infiltration in lymphedematous skin. ( a ) Representative low-power (upper images) and high-power (lower images) immunofluorescent staining (left panels) and quantification (right panel) of DAPI (blue) and CD4 + cells (pink) in normal, LE, and LE + tx biopsy specimens. Area in dotted box is shown in high-power views. ( b ) Representative low-power (upper images) and high-power (lower images) immunofluorescent staining (left panels) and quantification (right panel) of DAPI (blue), LYVE-1 (red), and tryptase (green) in normal, LE, and LE + tx biopsy specimens. Area in dotted box is shown in high-power views. Scale bar (low-power images): 200 μm; scale bar (magnified images): 25 μm; * p < 0.05; ** p < 0.01.

Techniques: Staining

Review

polyclonal goat igg anti mouse cd4 antibody (R&D Systems)

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1) Product Images from "Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease"

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